Impact
of Inhaled NO on Development of Neonatal Rat Lung
Theo Gs Đinh-Xuân Anh-Tuấn thì kết quả
nghiên cứu này sẽ được trình bày tại Hội Nghị Phổi và Lồng Ngực Hoa Kỳ
(American Thoracic Society) vào giữa tháng 5, 2008 tại Toronto
(http://www.thoracic.org/sections/meetings-and-courses/international-conference/2008/index.html).
Tác giả đầu (first authors) đều là các BS Việt Nam, hiện đang theo học
chương trình MD PhD tại Paris)
S.
Duong-Quy1, T. Hua-Huy1, P. Olivier2,
O. Baud2, J.C. Mercier3, and A.T. Dinh-Xuan1.
1Service
de Physiologie-Explorations Fonctionnelles,
Hôpital Cochin, France; 2INSERM Avenir
R05230HS, and 3Service des Urgences Pédiatriques, Hôpital Robert Debré,
Paris, France.
Rationale:
Inhaled nitric oxide (iNO) is administered to neonates in various clinical
settings in order to alleviate pulmonary hypertension whilst improving
oxygenation. However, the effect of iNO on fetal lung development has
not yet been investigated.
Objectives:
We assessed the effect of iNO on development of neonatal rat lungs.
Methods:
Pregnant rats were placed in a chamber containing 20 ppm NO and < 1
ppm NO2 starting from the last day of their pregnancy in order that keep
rat pups from birth (P0) to 7 days postnatal (P7). Control animals were
kept at room air. Rat pups were sacrificed at P7 and day 14 (P14) Body
and lung weights were measured at P7 and P14 in both groups. Lung vascular
density was evaluated by CD34 immunostaining (Santa Cruz) in paraffin-embedded
sections (left lung). VEGF expression of pulmonary arteries was semi-quantitatively
measured by immuno-peroxidase staining (Santa Cruz). Data was analyzed
on SPSS for Windows version 13.0 and Mann-Whitney Test was used for comparison
Results:
Lung weights did not differ between the two groups. Vascular density measured
at P7 and P14 remained also comparable in both groups. VEGF expression
assessed at P14 was however markedly reduced in the lungs from rats having
iNO as compared with controls (Fig).
Conclusion:
Inhalation of NO from birth (P0) to 7 days postnatal does not affect lung
weights or lung vascular density, as measured at P7 and P14, but significantly
reduces VEGF expression at P14. Whether this down regulating effect of
NO on VEGF expression is beneficial or detrimental on future rat lung
development remains unknown.
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